MYLIP Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9674R-Cy3 |
| Product Name | MYLIP Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human MYLIP |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 29116 |
| Swiss Prot | Q8WY64 |
| Subcellular location | Cell membrane |
| Synonyms | MIR; IDOL; E3 ubiquitin-protein ligase MYLIP; Inducible degrader of the LDL-receptor; Myosin regulatory light chain interacting protein; MYLIP; BZF1; BM-023; PP5242 |
| Background | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of myosin regulatory light chain (MRLC), LDLR, VLDLR and LRP8. Activity depends on E2 enzymes of the UBE2D family. Proteasomal degradation of MRLC leads to inhibit neurite outgrowth in presence of NGF by counteracting the stabilization of MRLC by saposin-like protein (CNPY2/MSAP) and reducing CNPY2-stimulated neurite outgrowth. Acts as a sterol-dependent inhibitor of cellular cholesterol uptake by mediating ubiquitination and subsequent degradation of LDLR. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is MYLIP Polyclonal Antibody, Cy3 Conjugated (bs-9674R-Cy3) validated for, and at what dilution?
bs-9674R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does MYLIP Polyclonal Antibody, Cy3 Conjugated (bs-9674R-Cy3) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Pig, Horse, Rabbit.
How should bs-9674R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise MYLIP Polyclonal Antibody, Cy3 Conjugated (bs-9674R-Cy3)?
KLH conjugated synthetic peptide derived from human MYLIP
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9674R-Cy3)?
Cy3 on bs-9674R-Cy3: excitation / emission 512,550nm/570,615nm.