Phospho-BLNK (Tyr84) Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9687R-Cy5.5 |
| Product Name | Phospho-BLNK (Tyr84) Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic phosphopeptide derived from human BLNK around the phosphorylation site of Tyr84 [EM(p-Y)VM] |
| Modification Site | Tyr84 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 29760 |
| Synonyms | BLNK phospho Y84; p-BLNK phospho Y84; B cell adapter containing SH2 domain protein; B cell adapter containing Src homology 2 domain protein; B cell linker; B cell linker protein; B cell linker protein; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; B-cell linker protein; BASH; BASH; Bca; Bca; BLNK; BLNK s; BLNK_HUMAN; Cytoplasmic adapter protein; Ly 57; Ly-57; Ly57; Lymphocyte antigen 57; Lymphocyte antigen-57; Lyw 57; Lyw-57; Lyw57; MGC111051; SLP 65; SLP65; SLP-65; Src homology 2 domain containing leukocyte protein of 65 kDa. |
| Background | This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, May 2012]. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is Phospho-BLNK (Tyr84) Polyclonal Antibody, Cy5.5 Conjugated (BS-9687R-CY5-5) validated for, and at what dilution?
BS-9687R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Phospho-BLNK (Tyr84) Polyclonal Antibody, Cy5.5 Conjugated (BS-9687R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-9687R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Phospho-BLNK (Tyr84) Polyclonal Antibody, Cy5.5 Conjugated (BS-9687R-CY5-5)?
KLH conjugated synthetic phosphopeptide derived from human BLNK around the phosphorylation site of Tyr84 [EM(p-Y)VM]
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-9687R-CY5-5)?
Cy5.5 on BS-9687R-CY5-5: excitation / emission 675nm/694nm.