HECA Polyclonal Antibody, AbBy Fluor-647 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9702R-BF647-100uL |
| Product Name | HECA Polyclonal Antibody, AbBy Fluor-647 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-647 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human HECA |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51696 |
| Swiss Prot | Q9UBI9 |
| Synonyms | HDCL; Gm869; HDC; HDC_HUMAN; Headcase homolog Drosophila; Headcase protein homolog; HECA; hHDC. |
| Background | HECA, also known as headcase homolog, HDC, HDCL or HHDC, is a 543 amino acid mammalian homolog of the Drosophila headcase protein, a highly basic, cytplasmic peptide that plays a role in mitotic re-entry during adult morphogenesis. Expressed in a variety of tissues with highest expression in thymus, spleen and heart, HECA is thought to play a role in the development of epithelial tube networks in lung tissue and may also be involved in the pathogenesis of lung cancer. The gene encoding HECA maps to human chromosome 6, which contains 170 million base pairs and comprises nearly 6% of the human genome. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Additionally, Porphyria cutanea tarda, Parkinson's disease, Stickler syndrome and a susceptibility to bipolar disorder are all associated with genes that map to chromosome 6. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is HECA Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9702R-BF647) validated for, and at what dilution?
bs-9702R-BF647 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HECA Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9702R-BF647) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9702R-BF647 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HECA Polyclonal Antibody, AbBy Fluor-647 Conjugated (bs-9702R-BF647)?
KLH conjugated synthetic peptide derived from human HECA
What are the excitation and emission wavelengths of the AbBy Fluor-647 conjugate (bs-9702R-BF647)?
AbBy Fluor-647 on bs-9702R-BF647: excitation / emission 650nm/665nm.