IFI35 Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9704R-RBITC-100uL |
| Product Name | IFI35 Polyclonal Antibody, RBITC Conjugated |
| Applications |
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human IFI35 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3430 |
| Synonyms | I 35; i-35; I35; P 35; P35; IN35; IN35_HUMAN; Interferon induced 35 kDa protein; Interferon-induced 35 kDa protein. |
| Background | The Interferon family of proteins are able to alter the expression of a variety of target genes, thereby controlling various events within the cell. IFI-35 (Interferon-induced 35 kDa protein), also known as IFP35, is a 286 amino acid interferon-induced protein. Localized to the nucleus and expressed in macrophages, fibroblasts and epithelial cells, IFI-35 is a leucine zipper protein that can form homodimers, but, unlike most leucine zipper proteins, cannot bind DNA. Upon induction by IFN-? IFI-35 associates with Nmi (N-Myc-interacting protein), resulting in the formation of a high molecular weight complex that is thought to play a role in IFN-?signaling and cellular responses. Once complexed with Nmi, IFI-35 is unable to be degraded by the proteasome, suggesting that IFI-35 is protected from degradation only when needed by IFN-? Two isoforms of IFI-35 exist due to alternative splicing events. |
| Application Dilution | |
| IF | WB1:300-5000 |
Frequently asked questions
Which applications is IFI35 Polyclonal Antibody, RBITC Conjugated (bs-9704R-RBITC) validated for, and at what dilution?
bs-9704R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does IFI35 Polyclonal Antibody, RBITC Conjugated (bs-9704R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9704R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise IFI35 Polyclonal Antibody, RBITC Conjugated (bs-9704R-RBITC)?
KLH conjugated synthetic peptide derived from human IFI35
What are the excitation and emission wavelengths of the RBITC conjugate (bs-9704R-RBITC)?
RBITC on bs-9704R-RBITC: excitation / emission 540nm/625nm.