ITM2A Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9705R-Cy5.5 |
| Product Name | ITM2A Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
FCM
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ITM2A |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 9452 |
| Swiss Prot | O43736 |
| Synonyms | E25A; BRICD2A; Integral membrane protein 2A; Protein E25; ITM2A; UNQ603/PRO1189 |
| Background | The type II integral membrane (ITM2) protein family consists of three members ITM2A (also designated E25), ITM2B and ITM2C. ITM2A expression is high in osteogenic and lymphoid tissues, while both ITM2B and ITM2C are expressed in brain. Mutations in the ITM2B gene can lead to familial British dementia (fbd), and autosomal dominant disease characterized by progressive dementia, spasticity, and cerebellar ataxia, or familial Danish dementia (fdd), an autosomal dominant disorder characterized by cataracts, deafness, progressive ataxia, and dementia. The ITM2A 263-amino acid protein contains an N-terminal cytosolic domain, an uncleaved signal anchor sequence, and a tyrosine-rich C-terminal domain. Human ITM2A shares 91% homology with mouse ITM2A. |
| Application Dilution | |
| WB | 1:300-5000 |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is ITM2A Polyclonal Antibody, Cy5.5 Conjugated (BS-9705R-CY5-5) validated for, and at what dilution?
BS-9705R-CY5-5 is validated for WB (1:300-5000); FCM (1:20-100); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ITM2A Polyclonal Antibody, Cy5.5 Conjugated (BS-9705R-CY5-5) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Pig, Rabbit.
How should BS-9705R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ITM2A Polyclonal Antibody, Cy5.5 Conjugated (BS-9705R-CY5-5)?
KLH conjugated synthetic peptide derived from human ITM2A
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-9705R-CY5-5)?
Cy5.5 on BS-9705R-CY5-5: excitation / emission 675nm/694nm.