NHEDC2 Polyclonal Antibody

U937 cell Liver lysates probed with NHEDC2 Polyclonal Antibody, Unconjugated (bs-9712R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

U937 cell Liver lysates probed with NHEDC2 Polyclonal Antibody, Unconjugated (bs-9712R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Mouse Spinal cord lysates; Lane 2: Mouse Kidney lysates probed with NHEDC2 Polyclonal Antibody, Unconjugated (bs-9712R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Lane 1: Mouse Spinal cord lysates; Lane 2: Mouse Kidney lysates probed with NHEDC2 Polyclonal Antibody, Unconjugated (bs-9712R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C

Lane 1: Human HL-60 cell lysates; Lane 2: Human Hcclm3 cell lysates; Lane 3: Human MOLT-4 cell lysates; Lane 4: Human U937 cell lysates probed with NHEDC2 Polyclonal Antibody, Unconjugated (bs-9712R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Lane 1: Human HL-60 cell lysates; Lane 2: Human Hcclm3 cell lysates; Lane 3: Human MOLT-4 cell lysates; Lane 4: Human U937 cell lysates probed with NHEDC2 Polyclonal Antibody, Unconjugated (bs-9712R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IF(IHC-P)

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-9712R
Product Name	NHEDC2 Polyclonal Antibody
Applications	WB, IHC-P, IF(IHC-P)
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic peptide derived from human NHEDC2
Immunogen Range	151-250/537
Clonality	Polyclonal
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	133308
Synonyms	NHA2; Mitochondrial Na+/H+ exchanger NHA2; Mitochondrial sodium/hydrogen exchanger NHA2; Na+/H+ exchanger like domain containing protein 2; Na+/H+ exchanger domain containing 2; NHE domain containing protein 2; NHE10; NHEDC 2; Sodium/hydrogen exchanger like domain containing protein 2; SL9B2_HUMAN.
Background	Na+/H+ exchangers (NHEs) catalyze the transport of Na+ in exchange for H+ across membranes in organisms and are required for numerous physiological processes. NHEDC2 (Na+/H+ exchanger-like domain-containing protein 2), also known as NHA2, is a 537 amino acid mitochondrial protein. NHEDC2 is involved in organelle volume homeostasis by catalyzing the exchange of protons for Na+ and Li+ across the inner mitochondrial membrane. Found in red blood cells, NHEDC2 is required for bone resorption activity and osteoclast differentiation. As a multi-pass membrane protein, NHEDC2 is expressed as two isoforms produced by alternative splicing events.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IF(IHC-P)	1:50-200

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