PDE4A8 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9719R-Cy3 |
| Product Name | PDE4A8 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human PDE4A8 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5141 |
| Synonyms | 2EL; DPDE2; PDE4; PDE46; PDE4A; Phosphodiesterase 4A; Phosphodiesterse Type 4A8; PDE4A_HUMAN. |
| Background | The PDE4A8 is a testis germ cell-specific PDE4A variant. There are two PDE4A variants identified in rat testis (PDE4A8 and an un-characterized 76 kDa PDE4A protein). Spermatogenesis is accompanied by expression of a varied repertoire of PDE4 enzymes at different stages of cell differentiation that presumably perform specialized functions. Confocal microscopic examination revealed the presence of PDE4A8 in primary and secondary spermatids but not in mature spermatozoa. PDE4A8 inhibition results in increased apoptosis of the germ cells accompanied with reduction in the number of mature spermatozoa. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is PDE4A8 Polyclonal Antibody, Cy3 Conjugated (bs-9719R-Cy3) validated for, and at what dilution?
bs-9719R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does PDE4A8 Polyclonal Antibody, Cy3 Conjugated (bs-9719R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9719R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise PDE4A8 Polyclonal Antibody, Cy3 Conjugated (bs-9719R-Cy3)?
KLH conjugated synthetic peptide derived from human PDE4A8
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9719R-Cy3)?
Cy3 on bs-9719R-Cy3: excitation / emission 512,550nm/570,615nm.