FAM29A Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9723R-Cy3 |
| Product Name | FAM29A Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human FAM29A/HAUS6 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Synonyms | Dgt6; Dim gamma tubulin homolog; Family with sequence similarity 29 member A; FLJ20060; HAUS augmin like complex subunit 6; HAUS6; KIAA15741; HAUS6_HUMAN. |
| Background | The human augmin complex (HAUS) is an evolutionarily conserved 8-subunit protein complex that was initially discovered in Drosophila. The HAUS complex is essential for microtubule generation, centrosome integrity, mitotic spindle assembly and completion of cytokinesis. HAUS6 (HAUS augmin-like complex, subunit 6), also known as FAM29A or Dgt6, is a 955 amino acid component of the augmin complex. Required for mitotic progression, HAUS6 localizes to cytoplasm, cytoskeleton, mitotic spindle microtubules and interphase centrosomes, and undergoes post-translational phosphorylation following mitosis on multiple serine and threonine residues. HAUS6 exists as two alternatively spliced isoforms that are encoded by a gene located on human chromosome 9. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is FAM29A Polyclonal Antibody, Cy3 Conjugated (bs-9723R-Cy3) validated for, and at what dilution?
bs-9723R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does FAM29A Polyclonal Antibody, Cy3 Conjugated (bs-9723R-Cy3) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rabbit.
How should bs-9723R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise FAM29A Polyclonal Antibody, Cy3 Conjugated (bs-9723R-Cy3)?
KLH conjugated synthetic peptide derived from human FAM29A/HAUS6
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9723R-Cy3)?
Cy3 on bs-9723R-Cy3: excitation / emission 512,550nm/570,615nm.