BIN2 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9727R-Cy5.5 |
| Product Name | BIN2 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human BIN2/BRAP1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 51411 |
| Synonyms | BIN2; BIN2_HUMAN; BRAP 1; Breast cancer associated protein BRAP1; Breast cancer-associated protein 1; Bridging integrator 2. |
| Background | BAR proteins are characterized by a common N-terminal BAR (bin, amphiphysin and Rvs161/167) domain and are recognized as adaptor proteins that are involved in many cellular processes. BIN1 and BIN2 are BAR proteins that share 61% sequence similarity. BIN1 (Bridging integrator 1) is a ubiquitously expressed regulatory protein for synaptic vesicle endocytosis. BIN1 also interacts with the transcription factors c-Myc and MyoD, potentially functioning as a tumor suppressor. BIN2, also known as Breast cancer-associated protein 1, is a 565 amino acid protein that interacts with BIN1. In contrast to BIN1, BIN2 lacks tumor suppressor features as well as a c-Myc interacting region. BIN2 shows preferred expression in tissues of hematopoietic origin, with high levels found in spleen, thymus, colon, placenta, lymphoid and granulocytic cells. There are two isoforms of BIN2 that are produced as a result of alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is BIN2 Polyclonal Antibody, Cy5.5 Conjugated (BS-9727R-CY5-5) validated for, and at what dilution?
BS-9727R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does BIN2 Polyclonal Antibody, Cy5.5 Conjugated (BS-9727R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-9727R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BIN2 Polyclonal Antibody, Cy5.5 Conjugated (BS-9727R-CY5-5)?
KLH conjugated synthetic peptide derived from human BIN2/BRAP1
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-9727R-CY5-5)?
Cy5.5 on BS-9727R-CY5-5: excitation / emission 675nm/694nm.