UFD1L Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9733R-Cy5.5-100uL |
| Product Name | UFD1L Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human UFD1L |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7353 |
| Synonyms | UB fusion protein 1; Ubiquitin fusion degradation 1 like yeast; Ubiquitin fusion degradation 1 like; Ubiquitin fusion degradation protein 1 homolog; UFD1; UFD1L. |
| Background | UFD1L is a member of the UFD1 family of proteins and is a component of the ubiquitin-dependent proteolytic pathway which degrades ubiquitin fusion proteins. This complex, also containing UFD1L, VCP and NPLOC4, binds ubiquitinated proteins and is required for the export of misfolded proteins from the ER to the cytoplasm for disposal. The NPLOC4-UFD1L-VCP complex also regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. UFD1L gene hemizygosity is the cause of some developmental defects including DiGeorge syndrome (DGS), velo-cardio-facial syndrome (VCFS) and Opitz G/BBB syndrome. UFD1L has 2 named isoforms produced by alternative splicing. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is UFD1L Polyclonal Antibody, Cy5.5 Conjugated (BS-9733R-CY5-5) validated for, and at what dilution?
BS-9733R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does UFD1L Polyclonal Antibody, Cy5.5 Conjugated (BS-9733R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-9733R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UFD1L Polyclonal Antibody, Cy5.5 Conjugated (BS-9733R-CY5-5)?
KLH conjugated synthetic peptide derived from human UFD1L
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-9733R-CY5-5)?
Cy5.5 on BS-9733R-CY5-5: excitation / emission 675nm/694nm.