TSEN2 Polyclonal Antibody, PE Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9735R-PE-100uL |
| Product Name | TSEN2 Polyclonal Antibody, PE Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Horse |
| Specifications | |
| Conjugation | PE |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human TSEN2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 80746 |
| Synonyms | HsSen2; MGC2776; MGC4440; SEN2; SEN2L; tRNA intron endonuclease Sen2; tRNA splicing endonuclease 2 homolog SEN2, S. cerevisiae; tRNA splicing endonuclease subunit Sen2; TSEN 2; SEN2_HUMAN. |
| Background | The tRNA-splicing endonuclease complex is responsible for identifying and cleaving pre-tRNA at both 5? and 3? splice sites, thereby releasing introns and free tRNA molecules with 2',3' cyclic phosphates and 5'-OH termini. In addition to its role in pre-tRNA splicing, the heterotetrameric endonuclease complex participates in mRNA processing and, via its association with pre-mRNA processing factors, is thought to link pre-tRNA and pre-mRNA splicing events. TSEN2 (tRNA-splicing endonuclease subunit Sen2), also known as tRNA-intron endonuclease Sen2, is a 465 amino acid nuclear protein that constitutes one of the two catalytic subunits of the tRNA-splicing endonuclease complex. There are three isoforms of TSEN2 that are produced as a result of alternative splicing events. Isoform 1 seems to carry the active site for 5?-splice site cleavage. Defects in the gene encoding TSEN2 are the cause of pontocerebellar hypoplasia type 2B, which is characterized by progressive microencephaly with epilepsy, extrapyramidal dyskinesia and chorea without spinal cord findings. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | |
Frequently asked questions
Which applications is TSEN2 Polyclonal Antibody, PE Conjugated (bs-9735R-PE) validated for, and at what dilution?
bs-9735R-PE is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does TSEN2 Polyclonal Antibody, PE Conjugated (bs-9735R-PE) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Horse.
How should bs-9735R-PE be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise TSEN2 Polyclonal Antibody, PE Conjugated (bs-9735R-PE)?
KLH conjugated synthetic peptide derived from human TSEN2
What are the excitation and emission wavelengths of the PE conjugate (bs-9735R-PE)?
PE on bs-9735R-PE: excitation / emission 496,564nm/578nm.