EDRF1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9737R-Cy5.5 |
| Product Name | EDRF1 Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human EDRF1/C10orf137 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 26098 |
| Synonyms | C10orf137 protein; Chromosome 10 open reading frame 137; EDRF1; Erythroid dferentiation related factor 1; EDRF1_HUMAN. |
| Background | The tetratricopeptide repeat (TPR) motif is a degenerate, 34 amino acid sequence found in many proteins and acts to mediate protein-protein interactions in various pathways. At the sequence level, there can be up to 16 tandem TPR repeats, each of which has a helix-turn-helix shape that stacks on other TPR repeats to achieve ligand binding specificity. EDRF1 (erythroid differentiation-related factor 1), also known as C10orf137 (chromosome 10 open reading frame 137), is a 1,238 amino acid protein containing two TPR repeats. Localizing to nucleus, EDRF1 is involved in transcriptional activation of globin genes by regulating DNA-binding activity of GATA-1 transcription factor. EDRF1 may also play an important role in organ development and histological differentiation. EDRF1 exists as four alternatively spliced isoforms and is encoded by a gene mapping to human chromosome 10q26.13. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is EDRF1 Polyclonal Antibody, Cy5.5 Conjugated (BS-9737R-CY5-5) validated for, and at what dilution?
BS-9737R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does EDRF1 Polyclonal Antibody, Cy5.5 Conjugated (BS-9737R-CY5-5) react with?
Tested reactivity: Human, Mouse, Rat.
How should BS-9737R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise EDRF1 Polyclonal Antibody, Cy5.5 Conjugated (BS-9737R-CY5-5)?
KLH conjugated synthetic peptide derived from human EDRF1/C10orf137
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-9737R-CY5-5)?
Cy5.5 on BS-9737R-CY5-5: excitation / emission 675nm/694nm.