ANKLE2 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9743R-Cy3-100uL |
| Product Name | ANKLE2 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse |
| Predicted Reactivity | Rat, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ANKLE2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23141 |
| Swiss Prot | Q86XL3 |
| Synonyms | ANKL2_HUMAN; ANKLE 2; ANKLE2; ankyrin repeat and LEM domain containing 2; Ankyrin repeat and LEM domain-containing protein 2; LEM domain containing 7; LEMD 7; LEMD7. |
| Background | Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. Mutations of ankyrin genes lead to severe genetic diseases such as fatal cardiac arrhythmias and hereditary spherocytosis. ANKLE2 (ankyrin repeat and LEM domain containing 2), also known as LEMD7, is a 938 amino acid single-pass membrane protein containing an ANK repeat and a LEM domain. Exsiting as two isoforms produced by alternative splicing events, the gene encoding ANKLE2 maps to human chromosome 12, which encodes over 1,100 genes and comprises approximately 4.5% of the human genome. Chromosome 12 is associated with a variety of diseases and afflictions, including hypochondrogenesis, achondrogenesis, Kniest dysplasia, Noonan syndrome and trisomy 12p, which causes facial developmental defects and seizure disorders. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is ANKLE2 Polyclonal Antibody, Cy3 Conjugated (bs-9743R-Cy3) validated for, and at what dilution?
bs-9743R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ANKLE2 Polyclonal Antibody, Cy3 Conjugated (bs-9743R-Cy3) react with?
Tested reactivity: Human, Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Sheep, Horse, Rabbit.
How should bs-9743R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ANKLE2 Polyclonal Antibody, Cy3 Conjugated (bs-9743R-Cy3)?
KLH conjugated synthetic peptide derived from human ANKLE2
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9743R-Cy3)?
Cy3 on bs-9743R-Cy3: excitation / emission 512,550nm/570,615nm.