BRD4/CAP Polyclonal Antibody, Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-9759R-Cy5.5 |
| Product Name | BRD4/CAP Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Cow, Pig, Horse, Chicken, Rabbit |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Brd4/CAP |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 23476 |
| Swiss Prot | O60885 |
| Synonyms | Brd4; BRD4_HUMAN; bromodomain containing protein 4; Bromodomain-containing protein 4; CAP; chromosome associated protein; HUNK1; Protein HUNK1. |
| Background | Brd4 belongs to the BET family, a group of structurally related proteins containing two bromodomains. Through these two domains, Brd4 associates with mitotic chromosomes and its expression correlates with cell growth. Expression of Brd4 inhibits cell cycle progression from G(1) to S, due to binding to the largest subunit of replication factor C (RFC) to prevent DNA elongation. Altered Brd4 function correlates with poorly differentiated carcinoma, with aggresive phenotype and a highly lethal outcome. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is BRD4/CAP Polyclonal Antibody, Cy5.5 Conjugated (BS-9759R-CY5-5) validated for, and at what dilution?
BS-9759R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-9759R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise BRD4/CAP Polyclonal Antibody, Cy5.5 Conjugated (BS-9759R-CY5-5)?
KLH conjugated synthetic peptide derived from human Brd4/CAP
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-9759R-CY5-5)?
Cy5.5 on BS-9759R-CY5-5: excitation / emission 675nm/694nm.