C1orf25 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9784R-Cy3 |
| Product Name | C1orf25 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Mouse |
| Predicted Reactivity | Human, Rat, Dog, Cow, Sheep, Horse, Rabbit |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human C1orf25 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 81627 |
| Synonyms | bG120K12.3; Chromosome 1 open reading frame 25; Hypothetical protein FLJ90512; MST070; MSTP070; N2 antibody N2 dimethylguanosine tRNA methyltransferase like; TRM1L_HUMAN; TRMT1-like protein; Trmt1l. |
| Background | C1orf25, also known as TRM1L (TRMT1-like protein), MST070 or MSTP070, is a 733 amino acid protein that belongs to the TRM1 family of proteins. Expressed in a wide variety of tissues, C1orf25 contains one C2H2-type zinc finger and shares sequence similarity with TRMT1, a tRNA methyltransferase that dimethylates guanine at position 26 of tRNAs. As is suggested by observations of C1orf25-deficient mice, C1orf25 is believed to play a role in exploratory behavior and motor coordination. The gene encoding C1orf25 localizes to chromosome 1 and, due to alternative splicing events, C1orf25 exists as at least two isoforms. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is C1orf25 Polyclonal Antibody, Cy3 Conjugated (bs-9784R-Cy3) validated for, and at what dilution?
bs-9784R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does C1orf25 Polyclonal Antibody, Cy3 Conjugated (bs-9784R-Cy3) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Human, Rat, Dog, Cow, Sheep, Horse, Rabbit.
How should bs-9784R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise C1orf25 Polyclonal Antibody, Cy3 Conjugated (bs-9784R-Cy3)?
KLH conjugated synthetic peptide derived from human C1orf25
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9784R-Cy3)?
Cy3 on bs-9784R-Cy3: excitation / emission 512,550nm/570,615nm.