C1orf31 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9785R-Cy3 |
| Product Name | C1orf31 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human C1orf31 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 388753 |
| Synonyms | C1orf31; CA031_HUMAN; Chromosome 1 open reading frame 31; Hypothetical protein LOC388753; RP5-827C21.3; Uncharacterized protein C1orf31. |
| Background | The cytochrome c oxidase (COX) family of proteins function as the final electron donor in the respiratory chain to drive a proton gradient across the inner mitochondrial membrane, ultimately resulting in the production of water. C1orf31 is a 125 amino acid mitochondrial protein that belongs to the cytochrome c oxidase subunit 6B family. There are three isoforms of C1orf31 that are produced as a result of alternative splicing events. The gene encoding C1orf31 maps to human chromosome 1, the largest human chromosome spanning about 260 million base pairs and making up 8% of the human genome. There are about 3,000 genes on chromosome 1, and considering the great number of genes there are also a large number of diseases associated with chromosome 1. Notably, the rare aging disease Hutchinson-Gilford progeria is associated with the LMNA gene which encodes lamin A. When defective, the LMNA gene product can build up in the nucleus and cause characteristic nuclear blebs. The mechanism of rapidly enhanced aging is unclear and is a topic of continuing exploration. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is C1orf31 Polyclonal Antibody, Cy3 Conjugated (bs-9785R-Cy3) validated for, and at what dilution?
bs-9785R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does C1orf31 Polyclonal Antibody, Cy3 Conjugated (bs-9785R-Cy3) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9785R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise C1orf31 Polyclonal Antibody, Cy3 Conjugated (bs-9785R-Cy3)?
KLH conjugated synthetic peptide derived from human C1orf31
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9785R-Cy3)?
Cy3 on bs-9785R-Cy3: excitation / emission 512,550nm/570,615nm.