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AFAP Polyclonal Antibody, HRP Conjugated

Applications

  • WB
  • IHC-P

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Dog
  • Cow
  • Sheep
  • Horse
Overview
Catalog # bs-9790R-HRP-100uL
Product Name AFAP Polyclonal Antibody, HRP Conjugated
Applications
WB IHC-P
Reactivity Human
Predicted Reactivity Mouse, Rat, Dog, Cow, Sheep, Horse
Specifications
Conjugation HRP
Host Rabbit
Source KLH conjugated synthetic peptide derived from human AFAP
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 60312
Swiss Prot Q8N556
Synonyms AFAP; AFAP110; AFAP-110; Actin filament-associated protein 1; 110 kDa actin filament-associated protein; AFAP1
Background Can cross-link actin filaments into both network and bundle structures (By similarity). May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
Application Dilution
WB 1:300-5000
IHC-P 1:200-400

Frequently asked questions

Which applications is AFAP Polyclonal Antibody, HRP Conjugated (bs-9790R-HRP) validated for, and at what dilution?

bs-9790R-HRP is validated for WB (1:300-5000); IHC-P (1:200-400). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does AFAP Polyclonal Antibody, HRP Conjugated (bs-9790R-HRP) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Dog, Cow, Sheep, Horse.

How should bs-9790R-HRP be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise AFAP Polyclonal Antibody, HRP Conjugated (bs-9790R-HRP)?

KLH conjugated synthetic peptide derived from human AFAP