ECRG4 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9807R-Cy5 |
| Product Name | ECRG4 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Rat |
| Predicted Reactivity | Mouse, Dog, Pig, Rabbit |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human ECRG4/C2orf40 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 84417 |
| Synonyms | AUGN_HUMAN; Augurin; C2orf40; Esophageal cancer-related gene 4 protein. |
| Background | ECRG4, also known as augurin or C2orf40, is a 148 amino acid secreted protein. Belonging to the augurin family, ECRG4 is thought to be a hormone. It has also been suggested that ECRG4 may act as a tumor suppressor. The gene that encodes ECRG4 maps to human chromosome 2, which consists of 237 million bases encoding over 1,400 genes, making up approximately 8% of the human genome. A number of genetic diseases are linked to genes on chromosome 2. Harlequin icthyosis, a rare and morbid skin deformity, is associated with mutations in the ABCA12 gene. The lipid metabolic disorder sitosterolemia is associated with ABCG5 and ABCG8. An extremely rare recessive genetic disorder, Alstr syndrome is due to mutations in the ALMS1 gene. Interestingly, chromosome 2 contains what appears to be a vestigial second centromere and vestigial telomeres which gives credence to the hypothesis that human chromosome 2 is the result of an ancient fusion of two ancestral chromosomes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is ECRG4 Polyclonal Antibody, Cy5 Conjugated (bs-9807R-Cy5) validated for, and at what dilution?
bs-9807R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ECRG4 Polyclonal Antibody, Cy5 Conjugated (bs-9807R-Cy5) react with?
Tested reactivity: Human, Rat. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Dog, Pig, Rabbit.
How should bs-9807R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ECRG4 Polyclonal Antibody, Cy5 Conjugated (bs-9807R-Cy5)?
KLH conjugated synthetic peptide derived from human ECRG4/C2orf40
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9807R-Cy5)?
Cy5 on bs-9807R-Cy5: excitation / emission 625,650nm/670nm.