C2ORF25 Polyclonal Antibody, Cy5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9808R-Cy5 |
| Product Name | C2ORF25 Polyclonal Antibody, Cy5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human C2ORF25 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 27249 |
| Synonyms | cblD; Chromosome 2 open reading frame 25; Protein C2orf25, mitochondrial; CL25022; Methylmalonic aciduria cobalamin deficiency cblD type, with homocystinuria; Protein C2orf25, mitochondrial; MMAD_HUMAN. |
| Background | C2ORF25 is a mitochondrial protein and its function is not fully identified. But, Vitamin B12 (cobalamin) is an essential cofactor in several metabolic pathways. Intracellular conversion of cobalamin to adenosylcobalamin in mitochondria and to methylcobalamin in cytoplasm is necessary for homeostasis of methylmalonic acid and homocysteine. C2ORF25 encodes a protein involved in an early step of cobalamin metabolism. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is C2ORF25 Polyclonal Antibody, Cy5 Conjugated (bs-9808R-Cy5) validated for, and at what dilution?
bs-9808R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does C2ORF25 Polyclonal Antibody, Cy5 Conjugated (bs-9808R-Cy5) react with?
Tested reactivity: Human.
How should bs-9808R-Cy5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise C2ORF25 Polyclonal Antibody, Cy5 Conjugated (bs-9808R-Cy5)?
KLH conjugated synthetic peptide derived from human C2ORF25
What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9808R-Cy5)?
Cy5 on bs-9808R-Cy5: excitation / emission 625,650nm/670nm.