phospho-LEF1 (Ser42) Polyclonal Antibody

Formalin-fixed and paraffin embedded human colon carcinoma labeled with Anti-phospho-LEF1(Ser42) Polyclonal Antibody, Unconjugated (bs-9842R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.

Lane 1: HL60 cell lysates probed with LEF1(Ser42) Polyclonal Antibody, Unconjugated (bs-9842R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

$Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Spleen tissue lysates probed with phospho-LEF1 (Ser42) Polyclonal Antibody, Unconjugated (bs-9842R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.$

Lane 1: Mouse Thymus tissue lysates; Lane 2: Mouse Spleen tissue lysates probed with phospho-LEF1 (Ser42) Polyclonal Antibody, Unconjugated (bs-9842R) at 1:1000 dilution and 4\u00b0C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

Applications

WB
IHC-P
IHC-F
IF

Reactivity

Human
Mouse
Rat

Overview
Catalog #	bs-9842R
Product Name	phospho-LEF1 (Ser42) Polyclonal Antibody
Applications	WB, IHC-P, IHC-F, IF
Specificity	This antibody will recognize LEF1(Ser40) in mouse and rat
Reactivity	Human, Mouse, Rat
Specifications
Conjugation	Unconjugated
Host	Rabbit
Source	KLH conjugated synthetic phosphopeptide derived from human LEF1 around the phosphorylation site of Ser42
Modification Site	Ser42
Clonality	Polyclonal
Clone #	#REF!
Isotype	IgG
Concentration	1ug/ul
Purification	Purified by Protein A.
Storage Buffer	0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition	Shipped at 4_. Store at -20_ for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	51176
Swiss Prot	Q9UJU2
Subcellular location	Nucleus
Synonyms	LEF-1; TCF10; TCF7L3; TCF1ALPHA; Lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF1-alpha; LEF1
Background	Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Application Dilution
WB	1:300-5000
IHC-P	1:200-400
IHC-F	IFIHC-P1:50-200
IF