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Streptokinase Polyclonal Antibody, APC-Cy7 Conjugated

Applications

  • IF(IHC-P)

Reactivity

  • Bacteria
Overview
Catalog # bs-9846R-APC-Cy7-100uL
Product Name Streptokinase Polyclonal Antibody, APC-Cy7 Conjugated
Applications
IF(IHC-P)
Reactivity Bacteria
Specifications
Conjugation APC-Cy7
Host Rabbit
Source KLH conjugated synthetic peptide derived from Streptococcus uberis PauA protein
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 24161944
Swiss Prot B9DW72
Subcellular location Extracellular, Secreted
Synonyms PauA; Plasminogen activator A; SUB1785; Streptokinase; Streptococcus uberis
Background Plasminogen activator A (PauA) is a Streptococcus uberis protein that functions similarly to proteins within the Staphylokinase/Streptokinase family. Streptococcus uberis is a common cause of infection and inflammatory disease in the bovine udder. Inflammation of breast tissue (mastitis) has been recognized as a serious threat to the welfare of the dairy cow and has a severe economic impact on the dairy industry. S. uberis has been shown to specifically activate bovine and ovine plasminogen and this is mediated by the extracellular protein, plasminogen activator uberis (PauA), which has a molecular mass of around 30 kDa. It has been postulated that activation of plasminogen by PauA may facilitate early bacterial colonization of the lactating gland by promoting the release of nutrients which are likely to be restricted in milk. (https://www.ncbi.nlm.nih.gov/pubmed/10483719).
Application Dilution
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is Streptokinase Polyclonal Antibody, APC-Cy7 Conjugated (bs-9846R-APC-Cy7) validated for, and at what dilution?

bs-9846R-APC-Cy7 is validated for IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Streptokinase Polyclonal Antibody, APC-Cy7 Conjugated (bs-9846R-APC-Cy7) react with?

Tested reactivity: Bacteria.

How should bs-9846R-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Streptokinase Polyclonal Antibody, APC-Cy7 Conjugated (bs-9846R-APC-Cy7)?

KLH conjugated synthetic peptide derived from Streptococcus uberis PauA protein

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bs-9846R-APC-Cy7)?

APC-Cy7 on bs-9846R-APC-Cy7: excitation / emission 650nm/780nm.