Titin Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9861R-BF488 |
| Product Name | Titin Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
FCM
IF(IHC-P)
|
| Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Cow, Pig |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Titin (Ig-like 15 domain) |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7273 |
| Swiss Prot | Q8WZ42 |
| Synonyms | TMD; CMH9; CMD1G; CMPD4; EOMFC; HMERF; MYLK5; LGMD2J; Titin; Connectin; Rhabdomyosarcoma antigen MU-RMS-40.14; TTN |
| Background | Key component in the assembly and functioning of vertebrate striated muscles. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase. |
| Application Dilution | |
| FCM | 1:20-100 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Titin Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9861R-BF488) validated for, and at what dilution?
bs-9861R-BF488 is validated for FCM (1:20-100); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Titin Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9861R-BF488) react with?
Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Cow, Pig.
How should bs-9861R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Titin Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9861R-BF488)?
KLH conjugated synthetic peptide derived from human Titin (Ig-like 15 domain)
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-9861R-BF488)?
AbBy Fluor-488 on bs-9861R-BF488: excitation / emission 499nm/519nm.