Sorcin Polyclonal Antibody, AbBy Fluor-680 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9871R-BF680 |
| Product Name | Sorcin Polyclonal Antibody, AbBy Fluor-680 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-680 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human SR1/Sorcin |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 6717 |
| Synonyms | 22 kDa protein; Calcium binding protein amplied in mutlidrug resistant cells; CP 22; CP-22; CP22; SCN; Sorcin class 4 gene; Sorcin; SORCN_HUMAN; SRI; V19. |
| Background | Sorcin is a highly conserved protein, with 95% homology between hamster and human sorcin sequences. Sorcin has four putative Ca-binding domains, two of which exhibit strong homology to calmodulin EF hand? motifs. Calcium binding directly to sorcin has been demonstrated by in vitro assays. Sorcin is closely related to members of calpain and sarcoplasmic Ca2+-binding protein subfamilies. Sorcin undergoes calcium-dependent translocation from the cytosol to cellular membranes. Sorcin binds to and modulates ryanodine receptors and is widely distributed including heart and brain tissues. At the subcellular level, sorcin localizes to T-tubule junctions of cardiac sarcoplasmic reticulum. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is Sorcin Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-9871R-BF680) validated for, and at what dilution?
bs-9871R-BF680 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Sorcin Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-9871R-BF680) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9871R-BF680 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Sorcin Polyclonal Antibody, AbBy Fluor-680 Conjugated (bs-9871R-BF680)?
KLH conjugated synthetic peptide derived from human SR1/Sorcin
What are the excitation and emission wavelengths of the AbBy Fluor-680 conjugate (bs-9871R-BF680)?
AbBy Fluor-680 on bs-9871R-BF680: excitation / emission 679nm/702nm.