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HBZ Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Rat
Overview
Catalog # bs-9883R-Cy5-100uL
Product Name HBZ Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF
Specificity Will cross-react with Hemoglobin subunit alpha (HBA)
Reactivity Human, Rat
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Hemoglobin zeta
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 3050
Swiss Prot P02008
Synonyms HBAZ; HBAZ_HUMAN; HBZ 2; HBZ; HBZ2; Hemoglobin subunit zeta; Hemoglobin zeta; Hemoglobin zeta chain; Zeta globin; Zeta-globin.
Background Zeta-globin is an alpha-like hemoglobin. The zeta-globin polypeptide is synthesized in the yolk sac of the early embryo, while alpha-globin is produced throughout fetal and adult life. The zeta-globin gene is a member of the human alpha-globin gene cluster that includes five functional genes and two pseudogenes. The order of genes is: 5' - zeta - pseudozeta - mu - pseudoalpha-1 - alpha-2 -alpha-1 - theta1 - 3'. [provided by RefSeq, Nov 2009].
Application Dilution
WB 1:300-5000
IF IHC-P1:50-200

Frequently asked questions

Which applications is HBZ Polyclonal Antibody, Cy5 Conjugated (bs-9883R-Cy5) validated for, and at what dilution?

bs-9883R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HBZ Polyclonal Antibody, Cy5 Conjugated (bs-9883R-Cy5) react with?

Tested reactivity: Human, Rat.

How should bs-9883R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HBZ Polyclonal Antibody, Cy5 Conjugated (bs-9883R-Cy5)?

KLH conjugated synthetic peptide derived from human Hemoglobin zeta

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9883R-Cy5)?

Cy5 on bs-9883R-Cy5: excitation / emission 625,650nm/670nm.

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