NDST1 Polyclonal Antibody, FITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9891R-FITC |
| Product Name | NDST1 Polyclonal Antibody, FITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | FITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human NDST1 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 3340 |
| Synonyms | [Heparan sulfate]-glucosamine N-sulfotransferase 1; Bunctional heparan sulfate N-deacetylase/N-sulfotransferase 1; Glucosaminyl N-deacetylase/N-sulfotransferase 1; Heparan sulfate N-deacetylase 1; Heparan sulfate N-sulfotransferase 1; HSNST 1; HSNST-1; HSST; HSST1; N-heparan sulfate sulfotransferase 1; N-HSST 1; NDST-1; Ndst1; NDST1_HUMAN. |
| Background | Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA dissacharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Compared to other NDST enzymes, its presence is absolutely required. Participates in biosynthesis of heparan sulfate that can ultimately serve as L-selectin ligands, thereby playing a role in inflammatory response. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is NDST1 Polyclonal Antibody, FITC Conjugated (bs-9891R-FITC) validated for, and at what dilution?
bs-9891R-FITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does NDST1 Polyclonal Antibody, FITC Conjugated (bs-9891R-FITC) react with?
Tested reactivity: Human.
How should bs-9891R-FITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise NDST1 Polyclonal Antibody, FITC Conjugated (bs-9891R-FITC)?
KLH conjugated synthetic peptide derived from human NDST1
What are the excitation and emission wavelengths of the FITC conjugate (bs-9891R-FITC)?
FITC on bs-9891R-FITC: excitation / emission 494nm/518nm.