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BPI Polyclonal Antibody, Cy5 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
Overview
Catalog # bs-9892R-Cy5
Product Name BPI Polyclonal Antibody, Cy5 Conjugated
Applications
WB IF
Reactivity Human
Specifications
Conjugation Cy5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human CAP57
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 671
Synonyms Neutrophil Bactericidal Protein BP30; Bactericidal permeability-increasing protein; Bactericidal/permeability increasing protein; BPI; BPI_HUMAN; CAP 57; CAP-57; CAP57.
Background The cytotoxic action of BPI is limited to many species of Gram-negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa.
Application Dilution
WB 1:300-5000
IF IHC-P1:50-200

Frequently asked questions

Which applications is BPI Polyclonal Antibody, Cy5 Conjugated (bs-9892R-Cy5) validated for, and at what dilution?

bs-9892R-Cy5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does BPI Polyclonal Antibody, Cy5 Conjugated (bs-9892R-Cy5) react with?

Tested reactivity: Human.

How should bs-9892R-Cy5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise BPI Polyclonal Antibody, Cy5 Conjugated (bs-9892R-Cy5)?

KLH conjugated synthetic peptide derived from human CAP57

What are the excitation and emission wavelengths of the Cy5 conjugate (bs-9892R-Cy5)?

Cy5 on bs-9892R-Cy5: excitation / emission 625,650nm/670nm.