Cappuccino Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9895R-RBITC-100uL |
| Product Name | Cappuccino Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CNO/Cappuccino |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Subcellular location | Cytoplasm |
| Synonyms | Cappuccino; Cappuccino homolog; Cappuccino homolog; FLJ11230; Protein cappuccino; Protein cappuccino homolog; BL1S4_HUMAN; Biogenesis of lysosome-related organelles complex 1 subunit 4; BLOC-1 subunit 4; BLOC1S4; BCAS4L; CNO. |
| Background | Biogenesis of lysosome-related organelles complex-1 (BLOC-1) is a multisubunit protein necessary for biogenesis of specialized organelles of the endosomal-lysosomal system (such as melanosomes and platelet-dense granules). The complex consists of coiled-coil-forming proteins Snapin, Pallidin, Cappuccino, Muted, BLOS1, BLOS2, and BLOS3. The localization of these proteins varies as they can be cytoplasmic, peripheral membrane bound or anchored to the vesicular membrane.Cappuccino, a primarily cytoplasmic protein, plays a role in the development of melanosomes, platelet-dense granules and other lysosome-related organelles. It interacts primarily with pallidin and Muted and has been implicated as an actin-nucleation factor that may play a role in crosstalk between microfilaments and microtubules. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is Cappuccino Polyclonal Antibody, RBITC Conjugated (bs-9895R-RBITC) validated for, and at what dilution?
bs-9895R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Cappuccino Polyclonal Antibody, RBITC Conjugated (bs-9895R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9895R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Cappuccino Polyclonal Antibody, RBITC Conjugated (bs-9895R-RBITC)?
KLH conjugated synthetic peptide derived from human CNO/Cappuccino
What are the excitation and emission wavelengths of the RBITC conjugate (bs-9895R-RBITC)?
RBITC on bs-9895R-RBITC: excitation / emission 540nm/625nm.