p47/Pleckstrin Polyclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9902R-RBITC-100uL |
| Product Name | p47/Pleckstrin Polyclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human Pleckstrin/p47 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 5341 |
| Synonyms | p47; Platelet 47 kDa protein; Platelet and leukocyte C kinase substrate; Platelet P47; Platelet p47 protein; Pleckstrin; PLEK; PLEK_HUMAN; FLJ27168; OTTHUMP00000159962. |
| Background | Platelet P47, also known as NSFL1C, UBX1, UBXD10 or UBXN2C, is a 370 amino acid protein that localizes to both the nucleus and the golgi apparatus (specifically to golgi stacks) and contains one SEP domain and one UBX domain. Functioning as part of a ternary complex with VCP (a protein involved in the heterotypic fusion of transport vesicles with their target membranes) and Syntaxin 5, p47 interacts with and reduces the ATPase activity of VCP and is required for the fragmentation of golgi stacks during mitosis and for subsequent reassembly of golgi stacks after mitosis. p47 is subject to phosphorylation during mitosis, which inhibits p47-golgi interaction and is, therefore, required for proper golgi stack formation and cisternal regrowth. Human p47 shares 89% sequence identity with its mouse counterpart, suggesting a conserved role between species. Multiple isoforms of p47 exist due to alternative splicing events. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is p47/Pleckstrin Polyclonal Antibody, RBITC Conjugated (bs-9902R-RBITC) validated for, and at what dilution?
bs-9902R-RBITC is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does p47/Pleckstrin Polyclonal Antibody, RBITC Conjugated (bs-9902R-RBITC) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9902R-RBITC be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise p47/Pleckstrin Polyclonal Antibody, RBITC Conjugated (bs-9902R-RBITC)?
KLH conjugated synthetic peptide derived from human Pleckstrin/p47
What are the excitation and emission wavelengths of the RBITC conjugate (bs-9902R-RBITC)?
RBITC on bs-9902R-RBITC: excitation / emission 540nm/625nm.