CPN2 Polyclonal Antibody, AbBy Fluor-488 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9907R-BF488 |
| Product Name | CPN2 Polyclonal Antibody, AbBy Fluor-488 Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-488 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CPN2 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1370 |
| Synonyms | CPN 2; CPN2; CPN-2; ACBP; Carboxypeptidase N 83 kDa chain; Carboxypeptidase N large subunit; Carboxypeptidase N regulatory subunit; Carboxypeptidase N subunit 2 [Precursor]; carboxypeptidase N, polypeptide 2; CPN2_HUMAN. |
| Background | CPN2 is a zinc metalloprotease, and cleaves carboxy-terminal arginines and lysines from peptides found in the bloodstream such as complement anaphylatoxins, kinins, and creatine kinase MM (CK-MM). By removing only one amino acid, CPN has the ability to change peptide activity and receptor binding. It is a 280 kDa tetrameric glycoprotein that is synthesized by the liver and secreted into the plasma. It consists of 2 identical 83 kDa regulatory subunits (CPN2) and 2 identical 50 kDa catalytic subunits (CPN1). CPN2, the 83 kDa subunit, binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit. CPN is a member of a larger family of carboxypeptidases, many of which also cleave arginine and lysine. Because of the highly conserved active sites and the possible redundant functions of carboxypeptidases, it has been difficult to elucidate the role of CPN in disease processes. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | IHC-P1:50-200 |
Frequently asked questions
Which applications is CPN2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9907R-BF488) validated for, and at what dilution?
bs-9907R-BF488 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CPN2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9907R-BF488) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9907R-BF488 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CPN2 Polyclonal Antibody, AbBy Fluor-488 Conjugated (bs-9907R-BF488)?
KLH conjugated synthetic peptide derived from human CPN2
What are the excitation and emission wavelengths of the AbBy Fluor-488 conjugate (bs-9907R-BF488)?
AbBy Fluor-488 on bs-9907R-BF488: excitation / emission 499nm/519nm.