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GPLD1 Polyclonal Antibody, Cy5.5 Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
  • Mouse
  • Rat
Overview
Catalog # bs-9910R-Cy5.5
Product Name GPLD1 Polyclonal Antibody, Cy5.5 Conjugated
Applications
WB IF
Reactivity Human, Mouse, Rat
Specifications
Conjugation Cy5.5
Host Rabbit
Source KLH conjugated synthetic peptide derived from human GPLD1/GPI-PLD
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 2822
Synonyms Glycoprotein phospholipase D; Glycosyl-phosphatidylinositol-specic phospholipase D; GPI-PLD; GPI-specic phospholipase D; GPIPLD; GPIPLDM; GPLD1; PHLD_HUMAN; Phosphatidylinositol glycan specic phospholipase D; Phosphatidylinositol-glycan-specic phospholipase D; PI-G PLD; PIGPLD; PIGPLD1.
Background Phosphatidylinositol-glycan-specific phospholipase D (GPI-PLD) is a high-density lipoprotein-associated protein found on chromosome 6p22 that specifically hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol-glycans (PI-Gs). GPI-PLD is found in serum, liver, cerebrospinal fluid and in milk. The majority of plasma GPI-PLD appears to be specifically associated with a small, discrete and minor fraction of lipoproteins containing apoA-I and apoA-IV. Serum GPI-PLD activity is reduced over 75% in systemic inflammatory response syndrome and the downregulation of GPI-PLD could play an important role in the control of proinflammatory responses.
Application Dilution
WB 1:300-5000
IF IHC-P1:50-200

Frequently asked questions

Which applications is GPLD1 Polyclonal Antibody, Cy5.5 Conjugated (BS-9910R-CY5-5) validated for, and at what dilution?

BS-9910R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does GPLD1 Polyclonal Antibody, Cy5.5 Conjugated (BS-9910R-CY5-5) react with?

Tested reactivity: Human, Mouse, Rat.

How should BS-9910R-CY5-5 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise GPLD1 Polyclonal Antibody, Cy5.5 Conjugated (BS-9910R-CY5-5)?

KLH conjugated synthetic peptide derived from human GPLD1/GPI-PLD

What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-9910R-CY5-5)?

Cy5.5 on BS-9910R-CY5-5: excitation / emission 675nm/694nm.