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TCN2 Polyclonal Antibody, Cy3 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Human

Predicted Reactivity

  • Mouse
  • Rat
  • Rabbit
Overview
Catalog # bs-9919R-Cy3
Product Name TCN2 Polyclonal Antibody, Cy3 Conjugated
Applications
WB IF(IHC-P)
Reactivity Human
Predicted Reactivity Mouse, Rat, Rabbit
Specifications
Conjugation Cy3
Host Rabbit
Source KLH conjugated synthetic peptide derived from human TCN2/Transcobalamin II
Clonality Polyclonal
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein A.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 6948
Synonyms Macrocytic anemia; TC; TC II; TC-2; TC2; TCII; TCN 2; TCO2_HUMAN; Transcobalamin 2; Transcobalamin II; Transcobalamin II; macrocytic anemia; Transcobalamin-2; Vitamin B12 binding protein 2.
Background Transcobalamin I (TCI) and Transcobalamin II (TCII) are secreted proteins belonging to the eukaryotic cobalamin transport proteins family and also to the vitamin B12-binding protein family. The genes encoding these proteins map to chromosome 11q11-q12 and 22q12.2, respectively. Transcobalamin I is a constituent of secondary granules in neutrophils, while Transcobalamin II binds cobalamin and mediates its transport into cells. These plasma proteins are expressed in various tissues and secretions.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is TCN2 Polyclonal Antibody, Cy3 Conjugated (bs-9919R-Cy3) validated for, and at what dilution?

bs-9919R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does TCN2 Polyclonal Antibody, Cy3 Conjugated (bs-9919R-Cy3) react with?

Tested reactivity: Human. Predicted reactivity (expected from sequence homology but not yet tested): Mouse, Rat, Rabbit.

How should bs-9919R-Cy3 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise TCN2 Polyclonal Antibody, Cy3 Conjugated (bs-9919R-Cy3)?

KLH conjugated synthetic peptide derived from human TCN2/Transcobalamin II

What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9919R-Cy3)?

Cy3 on bs-9919R-Cy3: excitation / emission 512,550nm/570,615nm.