CaMKII delta Polyclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bs-9921R-Cy5.5 |
| Product Name | CaMKII delta Polyclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Mouse |
| Predicted Reactivity | Rat, Cow, Pig |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human CAMK2D/CaMKII delta |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 817 |
| Synonyms | Calcium / calmodulin dependent protein kinase 2 delta; Calcium / calmodulin dependent protein kinase II delta; Calcium/calmodulin-dependent protein kinase type II subunit delta; CAM kinase 2 delta; CAM kinase II delta; CaM kinase II subunit delta; CAMK 2d; CaMK-II subunit delta; CAMK2D; CAMKD; CAMKI; KCC2D_HUMAN; RATCAMKI. |
| Background | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated å, , © and ∂, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but phosphorylation by a CaMK is also required for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory threonine residue at position 177. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is CaMKII delta Polyclonal Antibody, Cy5.5 Conjugated (BS-9921R-CY5-5) validated for, and at what dilution?
BS-9921R-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does CaMKII delta Polyclonal Antibody, Cy5.5 Conjugated (BS-9921R-CY5-5) react with?
Tested reactivity: Mouse. Predicted reactivity (expected from sequence homology but not yet tested): Rat, Cow, Pig.
How should BS-9921R-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise CaMKII delta Polyclonal Antibody, Cy5.5 Conjugated (BS-9921R-CY5-5)?
KLH conjugated synthetic peptide derived from human CAMK2D/CaMKII delta
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BS-9921R-CY5-5)?
Cy5.5 on BS-9921R-CY5-5: excitation / emission 675nm/694nm.