UVRAG Polyclonal Antibody, PerCP-Cy5.5 Conjugated
Applications
Predicted Reactivity
| Overview | |
| Catalog # | bs-9923R-PerCP-Cy5.5-100uL |
| Product Name | UVRAG Polyclonal Antibody, PerCP-Cy5.5 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Predicted Reactivity | Human, Mouse, Rat, Dog, Horse |
| Specifications | |
| Conjugation | PerCP-Cy5.5 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human UVRAG/DHTX |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 7405 |
| Synonyms | DHTX; Disrupted in heterotaxy; p63; UV radiation resistance associated; UV radiation resistance associated gene; UV radiation resistance associated gene protein; UV radiation resistance-associated gene protein; UVRAG; UVRAG_HUMAN. |
| Background | UVRAG, also known as p63 or DHTX, is a 699 amino acid cytoplasmic protein. UVRAG has been shown to activate the BECN1/PI 3-kinase complex, which promotes autophagy. Autophagy is the degradation of cellular proteins in the lysosomes, and when this pathway is suppressed, cell growth is deregulated. Mutations in the gene encoding UVRAG have been associated with colon cancer, suggesting that UVRAG is also involved in suppressing the proliferation and tumorigenicity of human colon cancer cells. UVRAG has been found to complement the ultraviolet sensitivity of xeroderma pigmentosum group C cells. Ubiquitously expressed, UVRAG is found at highest levels in kidney, lung, liver and brain. UVRAG contains one C2 domain, which is thought to be involved in calcium-dependent phospholipid binding. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is UVRAG Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-9923R-PERCP-CY5-5) validated for, and at what dilution?
BS-9923R-PERCP-CY5-5 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should BS-9923R-PERCP-CY5-5 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise UVRAG Polyclonal Antibody, PerCP-Cy5.5 Conjugated (BS-9923R-PERCP-CY5-5)?
KLH conjugated synthetic peptide derived from human UVRAG/DHTX
What are the excitation and emission wavelengths of the PerCP-Cy5.5 conjugate (BS-9923R-PERCP-CY5-5)?
PerCP-Cy5.5 on BS-9923R-PERCP-CY5-5: excitation / emission 482nm/694nm.