C12ORF42 Polyclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9948R-Cy3-100uL |
| Product Name | C12ORF42 Polyclonal Antibody, Cy3 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human C12ORF42 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 374470 |
| Synonyms | Chromosome 12 open reading frame 42; FLJ25323; Hypothetical protein LOC374470; MGC43592; MGC57409; Uncharacterized protein C12orf42; CL042_HUMAN. |
| Background | Encoding over 1,100 genes within 132 million bases, chromosome 12 makes up about 4.5% of the human genome. A number of skeletal deformities are linked to chromosome 12 including hypochondrogenesis, achondrogenesis and Kniest dysplasia. Noonan syndrome, which includes heart and facial developmental defects among the primary symptoms, is caused by a mutant form of PTPN11 gene product, SH-PTP2. Chromosome 12 is also home to a homeobox gene cluster which encodes crucial transcription factors for morphogenesis, and the natural killer complex gene cluster encoding C-type lectin proteins which mediate the NK cell response to MHC I interaction. Trisomy 12p leads to facial development defects, seizure disorders and a host of other symptoms varying in severity depending on the extent of mosaicism and is most severe in cases of complete trisomy. The C12orf42 gene product has been provisionally designated C12orf42 pending further characterization. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is C12ORF42 Polyclonal Antibody, Cy3 Conjugated (bs-9948R-Cy3) validated for, and at what dilution?
bs-9948R-Cy3 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does C12ORF42 Polyclonal Antibody, Cy3 Conjugated (bs-9948R-Cy3) react with?
Tested reactivity: Human.
How should bs-9948R-Cy3 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise C12ORF42 Polyclonal Antibody, Cy3 Conjugated (bs-9948R-Cy3)?
KLH conjugated synthetic peptide derived from human C12ORF42
What are the excitation and emission wavelengths of the Cy3 conjugate (bs-9948R-Cy3)?
Cy3 on bs-9948R-Cy3: excitation / emission 512,550nm/570,615nm.