LCA5/Lebercilin Polyclonal Antibody, AbBy Fluor-750 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bs-9973R-BF750-100uL |
| Product Name | LCA5/Lebercilin Polyclonal Antibody, AbBy Fluor-750 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Human, Mouse, Rat |
| Specifications | |
| Conjugation | AbBy Fluor-750 |
| Host | Rabbit |
| Source | KLH conjugated synthetic peptide derived from human LCA5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein A. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 167691 |
| Synonyms | C6orf152; LCA5; Leber congenital amaurosis 5; Leber congenital amaurosis 5 protein; ORF64; RGD1308555. |
| Background | Leber congenital amaurosis (LCA) is one of the most common causes of hereditary blindness or severe visual impairment in infants. Mutations in several genes with diverse functions mapping to two loci have been implicated in LCA causation. These proteins are involved in processes such as photoreceptor development and maintenance, phototransduction, vitamin A metabolism and protein trafficking. LCA5, also known as Lebercilin, is a ciliary protein that is widely expressed during development and localizes to the connecting cilia of photoreceptors and to the microtubules, centrioles and primary cilia of cultured mammalian cells. The Leber congenital amaurosis 5-like protein (LCA5L) is a 670 amino acid protein that belongs to the LCA5 family. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is LCA5/Lebercilin Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-9973R-BF750) validated for, and at what dilution?
bs-9973R-BF750 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does LCA5/Lebercilin Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-9973R-BF750) react with?
Tested reactivity: Human, Mouse, Rat.
How should bs-9973R-BF750 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise LCA5/Lebercilin Polyclonal Antibody, AbBy Fluor-750 Conjugated (bs-9973R-BF750)?
KLH conjugated synthetic peptide derived from human LCA5
What are the excitation and emission wavelengths of the AbBy Fluor-750 conjugate (bs-9973R-BF750)?
AbBy Fluor-750 on bs-9973R-BF750: excitation / emission 749nm/775nm.