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beta Amyloid (1-42) Monoclonal Antibody, PerCP-Cy7 Conjugated

Applications

  • WB
  • IF(IHC-P)

Reactivity

  • Rat

Predicted Reactivity

  • Human
  • Mouse
Overview
Catalog # bsm-0107m-percp-cy7-100uL
Product Name beta Amyloid (1-42) Monoclonal Antibody, PerCP-Cy7 Conjugated
Applications
WB IF(IHC-P)
Reactivity Rat
Predicted Reactivity Human, Mouse
Specifications
Conjugation PerCP-Cy7
Host Mouse
Source KLH conjugated synthetic peptide derived from human beta-Amyloid (1-42)
Clonality Monoclonal
Clone # 1F4
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein G.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 351
Background The cerebral and vascular plaques associated with Alzheimer's disease are mainly composed of Amyloid beta peptides. beta Amyloid is derived from cleavage of the Amyloid precursor protein and varies in length from 39 to 43 amino acids. beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides result from cleavage of Amyloid precursor protein after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last Amyloid precursor protein processing step. beta Amyloid [1-40], beta Amyloid [1-42], and beta Amyloid [1-43] peptides are major constituents of the plaques and tangles that occur in Alzheimer's disease. beta Amyloid and peptides have been developed as tools for elucidating the biology of Alzheimer's disease.
Application Dilution
WB 1:300-5000
IF(IHC-P) 1:50-200

Frequently asked questions

Which applications is beta Amyloid (1-42) Monoclonal Antibody, PerCP-Cy7 Conjugated (bsm-0107M-PerCP-Cy7) validated for, and at what dilution?

bsm-0107M-PerCP-Cy7 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does beta Amyloid (1-42) Monoclonal Antibody, PerCP-Cy7 Conjugated (bsm-0107M-PerCP-Cy7) react with?

Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse.

How should bsm-0107M-PerCP-Cy7 be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise beta Amyloid (1-42) Monoclonal Antibody, PerCP-Cy7 Conjugated (bsm-0107M-PerCP-Cy7)?

KLH conjugated synthetic peptide derived from human beta-Amyloid (1-42)

What are the excitation and emission wavelengths of the PerCP-Cy7 conjugate (bsm-0107M-PerCP-Cy7)?

PerCP-Cy7 on bsm-0107M-PerCP-Cy7: excitation / emission 482nm/780nm.