TNF-alpha (1F6) Monoclonal Antibody

Applications

  • WB
  • IHC-P
  • IF(IHC-P)

Reactivity

  • Human
Overview
Catalog # bsm-0387M
Product Name TNF-alpha (1F6) Monoclonal Antibody
Applications WB, IHC-P, IF(IHC-P)
Specificity This antibody is produced using full length recombinant TNF-alpha protein.
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source This antibody is produced using full length recombinant human TNF-alpha.
Clonality Monoclonal
Clone # 1F6
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein G.
Storage Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Gene ID 7124
Swiss Prot P01375
Subcellular location Secreted, Membrane bound
Synonyms DIF; TNFA; TNFSF2; TNF-alpha; Tumor necrosis factor; Cachectin; Tumor necrosis factor ligand superfamily member 2; TNF-a; TNF
Background Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Application Dilution
WB 1:300-1000
IHC-P 1:200-400
IF(IHC-P) 1:50-200