ET-1(2C4) (2C4) Monoclonal Antibody, AbBy Fluor-594 Conjugated
Applications
Reactivity
Predicted Reactivity
| Overview | |
| Catalog # | bsm-0954M-BF594-100uL |
| Product Name | ET-1(2C4) (2C4) Monoclonal Antibody, AbBy Fluor-594 Conjugated |
| Applications |
WB
IF(IHC-P)
|
| Reactivity | Rat |
| Predicted Reactivity | Human, Mouse, Dog, Cow, Sheep, Pig, Horse, GuineaPig |
| Specifications | |
| Conjugation | AbBy Fluor-594 |
| Host | Mouse |
| Source | KLH conjugated synthetic peptide derived from human Endothelin-1 |
| Clonality | Monoclonal |
| Clone # | 2C4 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 1906 |
| Synonyms | EDN 1; EDN1; EDN1 protein; Endothelin1; ET 1; ET1; |
| Background | Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF(IHC-P) | 1:50-200 |
Frequently asked questions
Which applications is ET-1(2C4) (2C4) Monoclonal Antibody, AbBy Fluor-594 Conjugated (bsm-0954M-BF594) validated for, and at what dilution?
bsm-0954M-BF594 is validated for WB (1:300-5000); IF(IHC-P) (1:50-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does ET-1(2C4) (2C4) Monoclonal Antibody, AbBy Fluor-594 Conjugated (bsm-0954M-BF594) react with?
Tested reactivity: Rat. Predicted reactivity (expected from sequence homology but not yet tested): Human, Mouse, Dog, Cow, Sheep, Pig, Horse, GuineaPig.
How should bsm-0954M-BF594 be stored?
Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise ET-1(2C4) (2C4) Monoclonal Antibody, AbBy Fluor-594 Conjugated (bsm-0954M-BF594)?
KLH conjugated synthetic peptide derived from human Endothelin-1
What are the excitation and emission wavelengths of the AbBy Fluor-594 conjugate (bsm-0954M-BF594)?
AbBy Fluor-594 on bsm-0954M-BF594: excitation / emission 590nm/617nm.