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HA tag (12CA5) Monoclonal Antibody

Applications

  • WB
  • IF(ICC)

Reactivity

  • Tags
Overview
Catalog # bsm-0966m-100uL
Product Name HA tag (12CA5) Monoclonal Antibody
Applications
WB IF(ICC)
Reactivity Tags
Specifications
Conjugation Unconjugated
Host Mouse
Source KLH conjugated to HA tag
Clonality Monoclonal
Clone # 12CA5
Isotype IgG
Concentration Lot Dependent
Purification Purified by Protein G.
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
Target
Background Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.
Application Dilution
WB 1:50-200
IF(ICC) 1:300-5000

Frequently asked questions

Which applications is HA tag (12CA5) Monoclonal Antibody (bsm-0966M) validated for, and at what dilution?

bsm-0966M is validated for WB (1:50-200); IF(ICC) (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does HA tag (12CA5) Monoclonal Antibody (bsm-0966M) react with?

Tested reactivity: Tags.

How should bsm-0966M be stored?

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise HA tag (12CA5) Monoclonal Antibody (bsm-0966M)?

KLH conjugated to HA tag

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