HA tag (12CA5) Monoclonal Antibody, Cy5.5 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-0966m-cy55-100l |
| Product Name | HA tag (12CA5) Monoclonal Antibody, Cy5.5 Conjugated |
| Applications |
WB
IF(ICC)
|
| Reactivity | Tags |
| Specifications | |
| Conjugation | Cy5.5 |
| Host | Mouse |
| Source | KLH conjugated to HA tag |
| Clonality | Monoclonal |
| Clone # | 12CA5 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Background | Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. |
| Application Dilution | |
| WB | 1:50-200 |
| IF(ICC) | 1:300-5000 |
Frequently asked questions
Which applications is HA tag (12CA5) Monoclonal Antibody, Cy5.5 Conjugated (BSM-0966M-CY5-5) validated for, and at what dilution?
BSM-0966M-CY5-5 is validated for WB (1:50-200); IF(ICC) (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does HA tag (12CA5) Monoclonal Antibody, Cy5.5 Conjugated (BSM-0966M-CY5-5) react with?
Tested reactivity: Tags.
How should BSM-0966M-CY5-5 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise HA tag (12CA5) Monoclonal Antibody, Cy5.5 Conjugated (BSM-0966M-CY5-5)?
KLH conjugated to HA tag
What are the excitation and emission wavelengths of the Cy5.5 conjugate (BSM-0966M-CY5-5)?
Cy5.5 on BSM-0966M-CY5-5: excitation / emission 675nm/694nm.