GAPDH(3E12) Monoclonal Antibody

Applications

  • WB

Reactivity

  • Human
  • Mouse
  • Rat
  • Sheep
  • Rabbit
Overview
Catalog # bsm-0978M
Product Name GAPDH(3E12) Monoclonal Antibody
Applications WB
Reactivity Human, Mouse, Rat, Sheep, Rabbit
Specifications
Conjugation Unconjugated
Host Mouse
Source full length GAPDH protein
Clonality Monoclonal
Clone # 3.00E+12
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein G.
Storage Aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store at -20°C for 12 months.
Target
Gene ID 2597
Swiss Prot P04406
Synonyms G3PD; GAPD; HEL-S-162eP; Glyceraldehyde-3-phosphate dehydrogenase; GAPDH; Peptidyl-cysteine S-nitrosylase GAPDH; CDABP47; OK/SW-cl.12
Background Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
Application Dilution
WB 1:300-1000