Bcl-2 Monoclonal Antibody, Cy3 Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-10846m-cy3 |
| Product Name | Bcl-2 Monoclonal Antibody, Cy3 Conjugated |
| Applications |
WB
|
| Reactivity | Human |
| Specifications | |
| Conjugation | Cy3 |
| Host | Mouse |
| Source | Recombinant protein fragment (1-220 amino acids range) of human Bcl-2. |
| Clonality | Monoclonal |
| Clone # | 1A5 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 596 |
| Swiss Prot | P10415 |
| Synonyms | Apoptosis regulator Bcl-2; BCL2 |
| Background | Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release |
| Application Dilution | |
| WB | 1:300-5000 |
Frequently asked questions
Which applications is Bcl-2 Monoclonal Antibody, Cy3 Conjugated (bsm-10846M-Cy3) validated for, and at what dilution?
bsm-10846M-Cy3 is validated for WB (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does Bcl-2 Monoclonal Antibody, Cy3 Conjugated (bsm-10846M-Cy3) react with?
Tested reactivity: Human.
How should bsm-10846M-Cy3 be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Bcl-2 Monoclonal Antibody, Cy3 Conjugated (bsm-10846M-Cy3)?
Recombinant protein fragment (1-220 amino acids range) of human Bcl-2.
What are the excitation and emission wavelengths of the Cy3 conjugate (bsm-10846M-Cy3)?
Cy3 on bsm-10846M-Cy3: excitation / emission 512,550nm/570,615nm.