AFP Monoclonal Antibody, RBITC Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-1621m-rbitc |
| Product Name | AFP Monoclonal Antibody, RBITC Conjugated |
| Applications |
WB
IF
|
| Reactivity | Human, Rat |
| Specifications | |
| Conjugation | RBITC |
| Host | Mouse |
| Source | Full length human AFP protein |
| Clonality | Monoclonal |
| Clone # | A2 |
| Isotype | IgG |
| Concentration | 1ug/ul |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. |
| Target | |
| Gene ID | 174 |
| Swiss Prot | P02771 |
| Synonyms | AFPD; FETA; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; AFP |
| Background | Alpha-fetoprotein (AFP) is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. |
| Application Dilution | |
| WB | 1:300-5000 |
| IF | |
Frequently asked questions
Which applications is AFP Monoclonal Antibody, RBITC Conjugated (bsm-1621M-RBITC) validated for, and at what dilution?
bsm-1621M-RBITC is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.
Which species does AFP Monoclonal Antibody, RBITC Conjugated (bsm-1621M-RBITC) react with?
Tested reactivity: Human, Rat.
How should bsm-1621M-RBITC be stored?
Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise AFP Monoclonal Antibody, RBITC Conjugated (bsm-1621M-RBITC)?
Full length human AFP protein
What are the excitation and emission wavelengths of the RBITC conjugate (bsm-1621M-RBITC)?
RBITC on bsm-1621M-RBITC: excitation / emission 570nm/595nm.