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AFP (A4) Monoclonal Antibody, RBITC Conjugated

Applications

  • WB
  • IF

Reactivity

  • Human
Overview
Catalog # bsm-1622m-rbitc
Product Name AFP (A4) Monoclonal Antibody, RBITC Conjugated
Applications
WB IF
Reactivity Human
Specifications
Conjugation RBITC
Host Mouse
Source Full-length AFP protein
Clonality Monoclonal
Clone # A4
Isotype IgG
Concentration 1ug/ul
Purification Purified by Protein G.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 174
Swiss Prot P02771
Synonyms AFPD; FETA; HPAFP; Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; AFP
Background Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.
Application Dilution
WB 1:300-5000
IF

Frequently asked questions

Which applications is AFP (A4) Monoclonal Antibody, RBITC Conjugated (bsm-1622M-RBITC) validated for, and at what dilution?

bsm-1622M-RBITC is validated for WB (1:300-5000); IF. These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does AFP (A4) Monoclonal Antibody, RBITC Conjugated (bsm-1622M-RBITC) react with?

Tested reactivity: Human.

How should bsm-1622M-RBITC be stored?

Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise AFP (A4) Monoclonal Antibody, RBITC Conjugated (bsm-1622M-RBITC)?

Full-length AFP protein

What are the excitation and emission wavelengths of the RBITC conjugate (bsm-1622M-RBITC)?

RBITC on bsm-1622M-RBITC: excitation / emission 570nm/595nm.