Metronidazole(1C2) Monoclonal Antibody, Biotin Conjugated
Applications
Reactivity
| Overview | |
| Catalog # | bsm-2059M-Biotin-100uL |
| Product Name | Metronidazole(1C2) Monoclonal Antibody, Biotin Conjugated |
| Applications |
ELISA
|
| Reactivity | Others |
| Specifications | |
| Conjugation | Biotin |
| Host | Mouse |
| Source | KLH conjugated with Metronidazole |
| Clonality | Monoclonal |
| Clone # | 1C2 |
| Isotype | IgG |
| Concentration | Lot Dependent |
| Purification | Purified by Protein G. |
| Storage Buffer | Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| Storage Condition | Store at -20°C for 12 months. |
| Target | |
| Synonyms | 2-Methyl-5-nitroimidazole-1-ethanol |
| Background | Metronidazole is a nitroimidazole antibiotic medication used particularly for anaerobic bacteria and protozoa. Metronidazole is an antibiotic, amebicide, and antiprotozoal.Metronidazole, taken up by diffusion, is selectively absorbed by anaerobic bacteria and sensitive protozoa. Once taken up by anaerobes, it is non-enzymatically reduced by reacting with reduced ferredoxin, which is generated by pyruvate oxido-reductase. Many of the reduced nitroso intermediates will form sulfinamides and thioether linkages with cysteine-bearing enzymes, thereby deactivating these critical enzymes. As many as 150 separate enzymes are affected.In addition or alternatively, the metronidazole metabolites are taken up into bacterial DNA, and form unstable molecules. This function only occurs when metronidazole is partially reduced, and because this reduction usually happens only in anaerobic cells, it has relatively little effect upon human cells or aerobic bacteria. |
| Application Dilution | |
| ELISA | 1:300-5000 |
Frequently asked questions
Which applications is Metronidazole(1C2) Monoclonal Antibody, Biotin Conjugated (bsm-2059M-Biotin) validated for, and at what dilution?
bsm-2059M-Biotin is validated for ELISA (1:300-5000). These are recommended starting dilutions; optimal dilutions should be determined by the end user.
How should bsm-2059M-Biotin be stored?
Store at -20°C for 12 months. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH 7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
What immunogen was used to raise Metronidazole(1C2) Monoclonal Antibody, Biotin Conjugated (bsm-2059M-Biotin)?
KLH conjugated with Metronidazole