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Ki-67 Recombinant Antibody

The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Ki-67 antibody (bsm-25816R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Rabbit Anti-Ki-67 antibody (bsm-25816R,1:100); Isotype Control (orange): Rabbit IgG (bs-0295P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • Flow-Cyt

Reactivity

  • Human
Overview
Catalog # bsm-25816r
Product Name Ki-67 Recombinant Antibody
Applications
FLOW-CYT
Reactivity Human
Specifications
Conjugation Unconjugated
Host Rabbit
Source KLH conjugated synthetic peptide derived from human Ki67
Clonality Recombinant
Isotype IgG
Concentration 1mg/ml
Purification Purified by Protein A.
Storage Buffer 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 4288
Swiss Prot P46013
Subcellular location Nucleus
Synonyms KIA; MIB-; MIB-1; PPP1R105; KI67_HUMAN; MKI67; Antigen identified by monoclonal antibody Ki-67 (Antigen KI-67 | Antigen Ki67);
Application Dilution
Flow-Cyt =1:100-200

Frequently asked questions

Which applications is Ki-67 Recombinant Antibody (bsm-25816R) validated for, and at what dilution?

bsm-25816R is validated for Flow-Cyt (Flow-Cyt=1:100-200). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does Ki-67 Recombinant Antibody (bsm-25816R) react with?

Tested reactivity: Human.

How should bsm-25816R be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise Ki-67 Recombinant Antibody (bsm-25816R)?

KLH conjugated synthetic peptide derived from human Ki67

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