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human CD15 (SSEA-1) Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (human CD15 (SSEA-1) ) Monoclonal Antibody, Unconjugated (bsm-30016M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human cervical carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (human CD15 (SSEA-1) ) Monoclonal Antibody, Unconjugated (bsm-30016M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (human CD15 (SSEA-1) ) Monoclonal Antibody, Unconjugated (bsm-30016M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37\u00b0C for 30min; Antibody incubation with (human CD15 (SSEA-1) ) Monoclonal Antibody, Unconjugated (bsm-30016M) at 1:200 overnight at 4\u00b0C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
scatter diagram showing peripheral blood lymphocytes stained with CD15\/SSEA1. The cells were incubated with the antibody (bsm-30016M) for 30 min at 22\u00b0C.The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.
scatter diagram showing peripheral blood lymphocytes stained with CD15\/SSEA1. The cells were incubated with the antibody (bsm-30016M) for 30 min at 22\u00b0C.The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.
Bioss unconjugated antibody vial

Applications

  • FCM

Reactivity

  • Human
Overview
Catalog # bsm-30016m-100uL
Product Name human CD15 (SSEA-1) Monoclonal Antibody
Applications
FCM
Reactivity Human
Specifications
Conjugation Unconjugated
Host Mouse
Source Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)
Clonality Monoclonal
Clone # 15F2
Isotype IgM
Concentration Lot Dependent
Purification Purified by Protein G.
Storage Buffer 0.01M TBS (pH7.4).
Storage Condition Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Synonyms Stage-Specific Embryonic Antigen 1, X-hapten, Lewis X, 3-FAL, CD15, SSEA1, SSEA-1; Sialyl-Lewis X;
Application Dilution
FCM 1:50-100

Frequently asked questions

Which applications is human CD15 (SSEA-1) Monoclonal Antibody (bsm-30016M) validated for, and at what dilution?

bsm-30016M is validated for FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does human CD15 (SSEA-1) Monoclonal Antibody (bsm-30016M) react with?

Tested reactivity: Human.

How should bsm-30016M be stored?

Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M TBS (pH7.4).

What immunogen was used to raise human CD15 (SSEA-1) Monoclonal Antibody (bsm-30016M)?

Mouse F9 Teratocarcinoma Stem Cells (X-irradiated)

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