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human CD4 (HIT4a) Monoclonal Antibody, APC-Cy7 Conjugated

Applications

  • FCM

Reactivity

  • Human
Overview
Catalog # bsm-30093M-APC-Cy7-100uL
Product Name human CD4 (HIT4a) Monoclonal Antibody, APC-Cy7 Conjugated
Applications
FCM
Reactivity Human
Specifications
Conjugation APC-Cy7
Host Mouse
Source KLH conjugated synthetic peptide derived from human CD4
Clonality Monoclonal
Clone # HIT4a
Isotype IgG2b
Concentration 1ug/ul
Purification Purified by Protein G.
Storage Buffer Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
Storage Condition Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
Target
Gene ID 920
Swiss Prot P01730
Subcellular location Cell membrane
Synonyms CD4 Molecule; T-Cell Surface Glycoprotein CD4; T-Cell Surface Antigen T4/Leu-3; CD4 Antigen (P55); CD4 Receptor; CD4 Antigen; CD4mut;
Background This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010].

Application Dilution
FCM 1:20-100

Frequently asked questions

Which applications is human CD4 (HIT4a) Monoclonal Antibody, APC-Cy7 Conjugated (bsm-30093M-APC-Cy7) validated for, and at what dilution?

bsm-30093M-APC-Cy7 is validated for FCM (1:20-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does human CD4 (HIT4a) Monoclonal Antibody, APC-Cy7 Conjugated (bsm-30093M-APC-Cy7) react with?

Tested reactivity: Human.

How should bsm-30093M-APC-Cy7 be stored?

Store at -20C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles. Storage buffer: Aqueous buffered solution containing 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

What immunogen was used to raise human CD4 (HIT4a) Monoclonal Antibody, APC-Cy7 Conjugated (bsm-30093M-APC-Cy7)?

KLH conjugated synthetic peptide derived from human CD4

What are the excitation and emission wavelengths of the APC-Cy7 conjugate (bsm-30093M-APC-Cy7)?

APC-Cy7 on bsm-30093M-APC-Cy7: excitation / emission 650nm/780nm.

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