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mouse Ly-6G/Ly-6C Monoclonal Antibody

Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with mouse Ly-6G\/Ly-6C Monoclonal Antibody, Unconjugated (bsm-30178A) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rat IgG antibody (green, bs-0293G-FITC), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse Spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with mouse Ly-6G\/Ly-6C Monoclonal Antibody, Unconjugated (bsm-30178A) at 1:200 overnight at 4\u00b0C. Followed by conjugated Goat Anti-Rat IgG antibody (green, bs-0293G-FITC), DAPI (blue, C02-04002) was used to stain the cell nuclei.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ly-6G Monoclonal Antibody, Unconjugated(bsm-30178A) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0293G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse lung; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ly-6G Monoclonal Antibody, Unconjugated(bsm-30178A) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0293G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ly-6G Monoclonal Antibody, Unconjugated(bsm-30178A) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0293G-HRP and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Mouse spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ly-6G Monoclonal Antibody, Unconjugated(bsm-30178A) at 1:200 overnight at 4\u00b0C, followed by conjugation to the bs-0293G-HRP and DAB (C-0010) staining.
Bioss unconjugated antibody vial

Applications

  • IHC-F
  • IF(IHC-P)
  • IHC-P
  • FCM

Reactivity

  • Mouse
Overview
Catalog # bsm-30178a-100uL
Product Name mouse Ly-6G/Ly-6C Monoclonal Antibody
Applications
IHC-F IF(IHC-P) IHC-P FCM
Reactivity Mouse
Specifications
Conjugation Unconjugated
Host Rat
Source Recombinant Mouse Ly-6G/Gr-1
Clonality Monoclonal
Clone # RB6-8C5
Isotype Rat IgG2b,k
Concentration Lot Dependent
Purification Purified by Protein G.
Storage Buffer 0.01M PBS.
Storage Condition Store at 2-8C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID 546644
Swiss Prot P35461
Synonyms Gr-1; Gr1; Ly 6G; Ly-6G.1; Ly6G; lymphocyte antigen 6 complex locus G; Lymphocyte antigen 6G.
Application Dilution
IHC-F 1:100-500
IF(IHC-P) IFIHC-P1:100-500
IHC-P IHC-P1:100-500
FCM FCM1:50-100

Frequently asked questions

Which applications is mouse Ly-6G/Ly-6C Monoclonal Antibody (bsm-30178A) validated for, and at what dilution?

bsm-30178A is validated for IHC-F(IHC-F) (1:100-500); IF(IHC-P)(IF(IHC-P)) (1:100-500); IHC-P(IHC-P) (1:100-500); FCM(FCM) (1:50-100). These are recommended starting dilutions; optimal dilutions should be determined by the end user.

Which species does mouse Ly-6G/Ly-6C Monoclonal Antibody (bsm-30178A) react with?

Tested reactivity: Mouse.

How should bsm-30178A be stored?

Store at 2-8C for one year. Avoid repeated freeze/thaw cycles. Storage buffer: 0.01M PBS.

What immunogen was used to raise mouse Ly-6G/Ly-6C Monoclonal Antibody (bsm-30178A)?

Recombinant Mouse Ly-6G/Gr-1

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