Human Bcl-2 Monoclonal Antibody

Name: Human Bcl-2 Monoclonal Antibody
Brand: Bioss
SKU: bsm-30235M
Price: 470.0 USD
Availability: InStock

$The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-Bcl-2 antibody (bsm-30235M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488(bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.$

The HeLa (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20\u2103,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.).Primary Antibody (green):Mouse Anti-Bcl-2 antibody (bsm-30235M): 1 \u03bcg\/10^6 cells; Secondary Antibody (white blue): Goat anti-Mouse IgG-BF488(bs-60296G-BF488): 1 \u03bcg\/test. Blank control (black): PBS. Acquisition of 20,000 events was performed.

$4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Bcl-2) monoclonal Antibody, unconjugated (bsm-30235M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.$

4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Bcl-2) monoclonal Antibody, unconjugated (bsm-30235M) 1:100, 90 min at 37\u00b0C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

$4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Bcl-2) monoclonal Antibody, unconjugated (bsm-30235M) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.$

4% Paraformaldehyde-fixed HeLa (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Bcl-2) monoclonal Antibody, unconjugated (bsm-30235M) 1:100, 90 min at 37\u00b0C; followed by BF488 conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-BF488) at 37\u00b0C for 90 min, then stained with Phalloidin\/BF555 for 30 min at room temperature; DAPI (blue, C02-04002) was used to stain the cell nuclei.

Applications

Flow-Cyt
ICC/IF

Reactivity

Human

Overview
Catalog #	bsm-30235m-100ul
Product Name	Human Bcl-2 Monoclonal Antibody
Applications	FLOW-CYT ICC/IF
Reactivity	Human
Specifications
Conjugation	Unconjugated
Host	Mouse
Clonality	Monoclonal
Clone #	8B4
Isotype	Mouse IgG2b, k
Concentration	Lot Dependent
Purification	Purified by Protein G.
Storage Buffer	0.01M TBS (pH7.4).
Storage Condition	Shipped at 4C. Store at -20C for one year. Avoid repeated freeze/thaw cycles.
Target
Gene ID	596
Subcellular location	Cytoplasm, Nucleus, Cell membrane
Synonyms	Bcl-2; PPP1R50; BCL2_HUMAN; BCL2; BCL2 apoptosis regulator; B-cell CLL/lymphoma 2; BCL2, apoptosis regulator; protein phosphatase 1, regulatory subunit 50
Application Dilution
Flow-Cyt	=1ug/Test
ICC/IF	ICC/IF=1:100-500

Recombinant Proteins (4).png__PID:b4703663-ad26-4eba-90ef-48f549942e61

bsm-30235m-100ul Copy

Data Sheet